Vector貨號(hào)H-1200，防熒光萃滅封片劑?
產(chǎn)品名稱：VECTASHIELD含有DAPI的封片劑
別名：VECTASHIELD Mounting Medium with DAPI
美國Vector labs產(chǎn)品貨號(hào)|編號(hào):Vector labs H-1200
Vector貨號(hào)H-1200，防熒光萃滅封片劑?
Vector H-1000:
Mounting Medium, 400張/10ml 防熒光萃滅效果尤好，長期保存。 FITC,Texas Red,Rhodamine,AMCN等。Vector H-1000實(shí)物圖見上圖。
Vector H-1200:
Mounting Medium with DAPI,含DAPI 400張/10ml ,防熒光萃滅；含DAPI,可襯染細(xì)胞核；用于ISH或襯染DNA的實(shí)驗(yàn)。 PE，F(xiàn)ITC等。Vector H-1200實(shí)物圖與Vector H-1000幾乎一樣，可參考上圖。
Vector H-1300:
含PI 400張/10ml ,防熒光萃滅；含PI，可襯染細(xì)胞核；用于ISH或襯染DNA的實(shí)驗(yàn)。 FITC等。
Vector H-1400:
HardSet Mounting Medium, 400張/10ml 凝固性；防熒光萃滅；適于油鏡觀察。 FITC,Texas Red,Rhodamine等。
Vector H-1500:
HardSet Mounting Medium with DAPI， 400張/10ml 凝固性；防熒光萃滅；含DAPI，可襯染細(xì)胞核，用于ISH或襯染DNA的實(shí)驗(yàn)；適于油鏡觀察。 FITC,Texas Red,含DAPI Rhodamine等。
Vector H-5000:
Permanent Mounting Medium ,240張/60ml 非水溶性，無毒、無味；清晰；和底物BCIP/NBT聯(lián)用，可將結(jié)晶減至zui小。長期保存。 HRP和AP的底物。
Vector H-5501:
AQ Aqueous Mounting Medium ,240張/60ml 水溶性，適于反應(yīng)產(chǎn)物為酒精或其它有機(jī)溶劑溶性的底物。 大多數(shù)HRP和AP的底物如AEC，DAB, BCIP/NBT, Vector SG.
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詳細(xì)信息：
VECTASHIELD® Mounting Media are unsurpassed in preventing photobleaching. The different formulations of VECTASHIELD® Mounting Media all offer the same outstanding anti-fade and anti-photobleaching properties. They are all compatible with fluorescein, Texas Red®, AMCA, DyLight® dyes, Alexa Fluor® dyes, fluorescent nuclear stains, fluorescent proteins, fluorescent tracers, histochemical stains, and most fluorochromes.
Features of VECTASHIELD® Mounting Media:
- Inhibit photobleaching of most fluorochromes
- Available in non-hardening or hardening formulations
- Available with or without DAPI or propidium iodide
- No warming necessary
- Slides can be viewed after prolonged storage
- Continues to inhibit photobleaching after prolonged storage of mounted slides
- Easy to use
- Ideal refractive index
The original VECTASHIELD® Mounting Medium is a glycerol-based, aqueous mountant that does not solidify but remains a viscous liquid on the slide. After mounting, coverslipped slides will not readily dry out and can be reviewed for weeks afterwards without sealing. For prolonged storage, coverslips can be permanently sealed around the perimeter with nail polish. Mounted slides should be stored at 4 ?C.
Both the VECTASHIELD® HardSet™ and the original VECTASHIELD® Mounting Media are available with or without the counterstain DAPI （4’, 6-diamidino-2-phenylindole）. DAPI produces a blue fluorescence when bound to DNA with excitation at about 360 nm and emission at 460 nm.
VECTASHIELD® Mounting Media are compatible with a wide array of fluorochromes, enzymatic substrates, and fluorescent proteins. Please consult the compatibility table （located in product information box） to determine if VECTASHIELD® will be compatible in your system.
Please note: VECTASHIELD® Mounting Medium may not be compatible with all enzymatic substrates or fluorescent proteins and test sections would be advisable when using anything other than commonly used fluorochromes.
The refractive index for VECTASHIELD® Mounting Medium is 1.44.
1R.J. Florijn, et. al. Cytometry, 19 （1995） 177-182.
VECTASHIELD® Mounting Medium Antifade Comparison
Other manufacturers measure the antifade properties of their mountants using labeled microspheres or arrayed spots. Vector Labs prefers to measure antifade properties of VECTASHIELD® mountants using frozen tissue sections immunohistochemically stained with fluorescently labeled secondary antibodies.
Antifade capability is measured using a 40x objective with real time imaging over 30 seconds of continuous exposure to the excitation illumination. Individual intensity measurements are recorded from 6 separate labeled regions and the average is calculated. The intensity after 30 second exposure is expressed as a percentage of the intensity at zero time. The values for PG are taken from the manufacturer’s published results.
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