Phosphate-Buffered Saline （PBS） is a balanced salt solution used for a variety of cell culture applications, such as washing cells before dissociation, transporting cells or tissue, diluting cells for counting, and preparing reagents. PBS is formulated without calcium and magnesium for rinsing chelators from the culture before cell dissociation. Life Technologies offers a variety of Gibco® PBS formulations for a range of cell culture applications.